SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of
SOLVED: a. Refer to the formula for calculating oligonucleotide melting temperatures presented on page 2 to estimate the melting temperature for the DNA hybrids formed between provided primers and the DNA template
